PKA alfa/beta CAT (fosfo Thr197) Antilichaam

Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.

5566

P17612

NP_002721.1

Human, Mouse, Rat

PKA alpha/beta CAT (phospho Thr197) antibody was raised against a peptide sequence around phosphorylation site of threonine 197 (R-T-W (p) -T-L) derived from Human PKA alpha/beta CAT.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

40 kDa

IgG

The antibody detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

PRKACA

Protein kinase, cAMP-dependent, catalytic, alpha

Homo sapiens

Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259.

W Li, Learn. Mem., Jan 1996; 2: 320 - 333.

Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959.

Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.

PRKACA , PRKACB, PKACA

WB, IHC, IF

4506055

Liquid