PKR Antilichaam

PKR Antibody: The interferon-inducible, double-stranded RNA (dsRNA) -dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

5610

P19525

NP_002750

Human, Rat

3949

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar PKR expression profile in human and mouse cell lines detected by two independent anti-PKR antibodies that recognize different epitopes, 3947 against C-terminus domain and 3949 against N-terminus domain. PKR proteins are detected in all the tested human cell lines except SK-N-SH cell line at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3949

Innate Immunity

PKR Antibody is affinity chromatography purified via peptide column.

Cat. No. 1208 - THP-1 cell lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Mouse (86%)

PKR Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

PKR antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human PKR has 2 isoforms, including isoform 1 (551aa, 62.1kD) and isoform S (510aa, 57.4kD. This antibody detects both human isoforms. Mouse PKR has 1 isoform (515aa, 58.3kD) . Rat PKR has only one isoform identified so far (513aa, 58.3kD) .

EIF2AK2

Cat. No. 1462 - Rat Lung Tissue Lysate

Cat. No. 1224 - Daudi Cell Lysate

Cat. No. 1202– A431 Cell Lysate

Eukaryotic translation initiation factor 2-alpha kinase 2

Homo sapiens

Meurs et al. Cell 1990; 62:379-90.

Saelens et al. J. Biol. Chem. 2001; 276:41620-8.

Gil and Esteban. Apoptosis 2000; 5:107-14.

Gil et al. FEBS Lett. 2002; 19:3665-74.

PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2

ELISA, WB, IHC-P

31747519

Liquid