PLKK (fosfo Ser482 / Ser486 / Ser490) Antilichaam

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490) . Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

380481

P70032

P70032

Frog

PLKK (Ser482/486/490) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the Xenopus Phospho-Ser482, 486, 490 PLKK.

Unconjugated

Primary Antibodies

Signal Transduction, Phospho-Specific

Affinity Purified

Batch dependent

Phospho-Specific

Blue Ice

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

120

PLKK antibody is specific for PLKK phosphorylated at Ser482,486,490.

Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not yet been determined. Xenopus is the only species tested to date. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Plk1

Polo-like kinase 1

Xenopus laevis

Van Vugt, M.A.T.M., van de Weerdt, B.C.M., Vader, G., Janssen, H., Calafat, J., Klompmaker, R., Wolthuis, R.M.F., and Medema, R.H., "Polo-like Kinase-1 Is Required for Bipolar Spindle Formation but Is Dispensable for Anaphase Promoting Complex/Cdc20 Activation and Initiation of Cytokinesis, " J. Biol. Chem., 279 (2004) 36,841-36,854.

Erikson, E., Haystead, T.A.J., Qian, Y-W., and Maller, J.L., "A Feedback Loop in the Polo-like Kinase Activation Pathway, " J. Biol. Chem., 279 (2004) 32,219-32,224.

Liu, J., Lewellyn, A.L., Chen, L.G., and Maller, J.L., "The Polo Box Is Required for Multiple Functions of Plx1 in Mitosis, " J. Biol. Chem., 279 (2004) 21,367 - 21,373.

Kumagai, A., and Dunphy, W. G., "Purification and Molecular Cloning of Plx1, a Cdc25-Regulatory Kinase from Xenopus Egg Extracts, " Science 273 (1996) 1377-1380.

WB

49036444

Liquid