PLOD1 Antilichaam / Lysylhydroxylase 1 / LH1

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.

Western blot: 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL

Q02809

Human

An E.coli-derived human recombinant protein (amino acids K23-A640) was used as the immunogen for the PLOD1 antibody.

WB, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This PLOD1 antibody is available for research use only.

After reconstitution, the PLOD1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the PLOD1 antibody should be determined by the researcher.

Western blot testing of human 1) U-87 MG, 2) HepG2 and 3) U-251 cell lysate with PLOD1 antibody. Predicted molecular weight ~83 kDa.