PNGase F-Fast

PNGase F-Fast is a glycosidase that catalyzes the cleavage of internal glycosidic bonds in oligosaccharides. PNGase F-Fast removes almost all N-linked oligosaccharides from glycoproteins. PNGase F-Fast can release N-glycans from glycoproteins in the sugar analysis workflow. The cleavage site is: the glycosidic bond between the innermost N-acetylglucosamine and asparagine. PNGase F-Fast is an improved reagent that allows for rapid deglycosylation of antibodies and antibody fusions within minutes[1][2].

83534-39-8

12352204

H334

Glycosidase

Metabolic Enzyme/Protease

Cancer-programmed cell death

Cancer

0

[PNGaseF-Fast]

H334

[1]Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021 Jan 9;31 (1) :2-7. |[2]Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015 Oct 20;87 (20) :10199-204.

Blue Ice

Store at -20°C for 2 years

http://file.medchemexpress.com/batch_PDF/HY-P2929A/PNGase-F-Fast-SDS-MedChemExpress.pdf

Enzyme

No Development Reported