POLM Antilichaam / DNA polymerase mu

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, ELISA: 0.1-0.5 µg/mL

Q9NP87

Human

E.coli-derived human POLM recombinant protein (Position: E36-H481) was used as the immunogen for the POLM antibody.

WB, IP, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This POLM antibody is available for research use only.

After reconstitution, the POLM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the POLM antibody should be determined by the researcher.

Immunoprecipitating POLM in 293T whole cell lysate. Western blot analysis of POLM using anti-POLM antibody. Lane 1: 293T whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-POLM antibody in 293T whole cell lysate, Lane 3: anti-POLM antibody (2ug) + 293T whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLM antibody at a dilution of 0.5 ug/ml and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for POLM at approximately 55 kDa. The expected molecular weight of POLM is ~55 kDa.