POLR2D cDNA

POLR2D encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from POLR2D.

HSRBP4, HSRPB4, RBP4, RPB16

2q21

Human

PATGen (puc19-derived cloning vector)

ATGGCGGCGGGTGGCAGCGATCCGCGGGCTGGCGACGTAGAGGAGGACGCCTCACAGCTCATCTTTCCTAAAGAGTTTGAAACAGCTGAGACACTTCTAAATTCAGAAGTTCATATGCTTCTGGAACATCGAAAGCAGCAGAATGAGAGTGCAGAGGACGAACAGGAGCTCTCAGAAGTCTTCATGAAAACATTAAACTACACAGCCCGTTTCAGTCGTTTCAAAAACAGAGAGACCATTGCCAGTGTTCGTAGCTTGCTACTCCAGAAAAAGCTTCATAAGTTTGAGTTGGCCTGTTTGGCCAACCTTTGCCCAGAGACTGCTGAGGAGTCCAAGGCTCTAATCCCAAGCTTGGAGGGACGGTTTGAAGATGAGGAGCTGCAGCAGATTCTTGATGATATCCAGACAAAGCGCAGCTTTCAGTATTAA

POLR2D, HSRBP4, HSRPB4, RBP4, RPB16, POLR2D, ATGD0477-10 µg, ATGD0477-20 µg, ATGD0477-50 µg, ATGD0477-100 µg, ATGD0477-250 µg, ATGD0477-500 µg, ATGD0477-1 mg, ATGD0477-10, ATGD0477-20, ATGD0477-50, ATGD0477-100, ATGD0477-250, ATGD0477-500, ATGD0477-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

606017

NP_004796.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_004805.3

429bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.