POLR2I cDNA

POLR2I encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [

HRPB14.5, RPB9

19q12

Human

PATGen (puc19-derived cloning vector)

ATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGTGGGTATTCGCTTCTGCCAGGAATGTAACAACATGCTGTACCCCAAGGAAGACAAGGAGAACCGCATTCTGCTCTACGCGTGCCGGAACTGTGATTACCAGCAGGAGGCCGACAACAGCTGCATCTATGTCAACAAGATCACGCACGAAGTGGACGAACTGACCCAGATTATCGCCGACGTGTCCCAGGACCCCACGTTGCCGCGGACCGAGGACCACCCGTGCCAAAAGTGCGGCCACAAGGAGGCTGTGTTCTTCCAGTCACACAGTGCGCGGGCCGAGGACGCCATGCGCCTTTACTACGTGTGCACAGCCCCACACTGCGGCCACCGCTGGACCGAGTGA

POLR2I, hRPB14.5, RPB9, POLR2I, ATGD0299-10 µg, ATGD0299-20 µg, ATGD0299-50 µg, ATGD0299-100 µg, ATGD0299-250 µg, ATGD0299-500 µg, ATGD0299-1 mg, ATGD0299-10, ATGD0299-20, ATGD0299-50, ATGD0299-100, ATGD0299-250, ATGD0299-500, ATGD0299-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

180662

NP_006224.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_006233.4

378bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.