POLR2J cDNA

POLR2J encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22. 1 and a pseudogene is found on chromosome 7p13.

HRPB14, POLR2J1, RPB11, RPB11A, RPB11m

7q22.1

Human

PATGen (puc19-derived cloning vector)

ATGAACGCCCCTCCAGCCTTCGAGTCGTTCTTGCTCTTCGAGGGCGAGAAGAAGATCACCATTAACAAGGACACCAAGGTACCCAATGCCTGTTTATTCACCATCAACAAAGAAGACCACACACTGGGAAACATCATTAAATCACAACTCCTAAAAGACCCGCAAGTGCTATTTGCTGGCTACAAAGTCCCCCACCCCTTGGAGCACAAGATCATCATCCGAGTGCAGACCACGCCGGACTACAGCCCCCAGGAAGCCTTTACCAACGCCATCACCGACCTCATCAGTGAGCTGTCCCTGCTGGAGGAGCGCTTTCGGGTGGCCATAAAAGACAAGCAGGAAGGAATTGAGTAG

POLR2J, hRPB14, POLR2J1, RPB11, RPB11A, RPB11m, POLR2J, ATGD0300-10 µg, ATGD0300-20 µg, ATGD0300-50 µg, ATGD0300-100 µg, ATGD0300-250 µg, ATGD0300-500 µg, ATGD0300-1 mg, ATGD0300-10, ATGD0300-20, ATGD0300-50, ATGD0300-100, ATGD0300-250, ATGD0300-500, ATGD0300-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

604150

NP_006225.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_006234.4

354bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.