POU5F1 Antilichaam

POU5F1 Antibody: POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

5460

Q01860

NP_002692

Human, Mouse, Rat

Unconjugated

Primary Antibodies

Homeostasis, Stem Cell

POU5F1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1404 - Mouse Liver Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)

POU5F1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

POU5F1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

POU5F1

POU class 5 homeobox 1

Homo sapiens

Scholer HR, Ruppert S, Suzuki N, et al. New type of POU domain in germ line-specific protein Oct-4. Nature1990; 344:435-9.

Scholer HR, Dressler GR, Balling R, et al. Oct-4: a germline-specific transcription factor mapping to the mouse t-complex. EMBO J.1990; 9:2185-95.

Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.

Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell2006; 1263:663-76.

POU5F1 Antibody: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4, OCT3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, 41915

ELISA, WB, IHC-P

42560248

Liquid