POU6F1 cDNA

POU6F1 belongs to the POu transcription factor family. This protein is transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3') . In the embryo, it is expressed exclusively in the developing brain, whereas in the adult its expression is restricted to brain, heart, skeletal muscle and lung. In the brain, the highest expression levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.

BRN5, MPOU, TCFB1

12q13.13

Human

PATGen (puc19-derived cloning vector)

ATGCCAGGGATCAGCAGTCAGATCCTCACCAATGCTCAGGGACAGGTTATTGGAACCCTTCCATGGGTAGTGAACTCAGCTAGTGTGGCGGCCCCAGCACCAGCCCAAAGCCTGCAGGTCCAGGCCGTGACCCCCCAGCTGTTGTTGAACGCCCAGGGCCAGGTGATTGCGACCCTGGCTAGCAGCCCCCTGCCTCCACCTGTGGCTGTCCGGAAGCCAAGCACACCTGAGTCCCCTGCTAAGAGTGAGGTGCAGCCCATCCAGCCCACACCAACCGTGCCCCAGCCTGCTGTGGTCATTGCCAGCCCAGCTCCAGCCGCCAAGCCATCTGCCTCTGCTCCTATCCCAATTACCTGCTCAGAGACCCCCACCGTCAGCCAGTTGGTGTCCAAGCCACATACTCCAAGTCTGGATGAGGATGGGATCAACTTAGAAGAGATCCGGGAGTTTGCCAAGAACTTTAAGATCCGGCGGCTCTCGCTGGGCCTTACACAGACCCAGGTGGGTCAGGCTCTGACTGCAACGGAAGGTCCAGCCTACAGCCAGTCAGCCATCTGCCGGTTCGAGAAGCTAGACATCACACCCAAGAGTGCCCAGAAGCTAAAGCCGGTGCTGGAAAAGTGGCTAAACGAAGCTGAACTGCGGAACCAGGAAGGCCAGCAGAACCTGATGGAGTTTGTGGGAGGCGAGCCCTCCAAGAAACGCAAACGCCGCACCTCCTTCACCCCCCAGGCCATAGAGGCTCTCAATGCCTATTTTGAGAAGAACCCACTGCCCACAGGCCAGGAGATCACTGAAATTGCTAAGGAGCTCAACTACGACCGTGAGGTAGTGCGGGTCTGGTTCTGCAATCGGCGCCAGACGCTCAAGAACACCAGCAAGCTGAACGTCTTTCAGATCCCTTAG

POU6F1, BRN5, MPOU, TCFB1, POU6F1, ATGD0487-10 µg, ATGD0487-20 µg, ATGD0487-50 µg, ATGD0487-100 µg, ATGD0487-250 µg, ATGD0487-500 µg, ATGD0487-1 mg, ATGD0487-10, ATGD0487-20, ATGD0487-50, ATGD0487-100, ATGD0487-250, ATGD0487-500, ATGD0487-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

NP_002693.3

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_002702.3

906bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.