PPP1R12A Antilichaam

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP) -bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

4659

O14974

Human

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PPP1R12A (NP_002471.1) .

Unconjugated

Primary Antibodies

Cell Cycle, Signal Transduction

Affinity purification

U-87MG

Batch dependent

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

None

Blue Ice

Store at -20˚ C. Avoid freeze / thaw cycles.

Observed: 140kDa

IgG

PPP1R12A

LO2

Jurkat

HeLa

Protein phosphatase 1 regulatory subunit 12A

Homo sapiens

M13MBS, MYPT1, protein phosphatase 1 regulatory subunit 12A, myosin binding subunit, myosin phosphatase, target subunit 1, myosin phosphatase-targeting subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A, protein phosphatase myosin-binding subunit

WB, IP

Liquid