PRAK Antilichaam (7H10B4]

PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK) -activated protein kinase (MAPKAPK) -5, is an ubiquitously expressed serine/threonine kinase regulated by p38α and p38β MAP kinases. Activated JNK, p38γ or p38δ are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.

8550

Q8IW41

Q8IW41

Human

Ni-NTA purified truncated recombinant PRAK-His expressed in E. Coli strain BL21 (DE3) .

7H10B4

Unconjugated

Primary Antibodies

Other

Batch dependent

Ascitic fluid containing 0.03% sodium azide.

None

Blue Ice

PRAK monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG1

MAPKAPK5

Mitogen-activated protein kinase-activated protein kinase 5

Homo sapiens

Paliga AJ. Natale DR. Watson AJ. Biol Cell. 2005, Aug, 97 (8) :629-40.

Wijtten PJ. Prak R. Lemme A. et al. Br Poult Sci. 2004, Aug, 45 (4) :504-11.

New L. Jiang Y. Han J. Mol Biol Cell. 2003, Jun, 14 (6) :2603-16. Epub 2003 Mar 20.

PRAKMAPK-activated protein kinase 5, MK5, MK-5, PRAK, MAPKAP-K5

ELISA, WB, IHC

52000829