PRKAR1A Antilichaam

CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC) . This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40) . Several alternatively spliced transcript variants encoding two different isoforms have been observed.

5573

P10644

Human

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PRKAR1A (NP_002725.1) .

Unconjugated

Primary Antibodies

Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction

Affinity purification

HeLa

Batch dependent

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

None

Blue Ice

Store at -20˚ C. Avoid freeze / thaw cycles.

Observed: 45kDa

IgG

PRKAR1A

PC-3

U-251MG

Protein kinase cAMP-dependent type I regulatory subunit alpha

Homo sapiens

ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, TSE1, cAMP-dependent protein kinase type I-alpha regulatory subunit, Carney complex type 1, cAMP-dependent protein kinase regulatory subunit RIalpha, cAMP-dependent protein kinase type I-alpha regulatory chain, epididymis secretory sperm binding protein, protein kinase A type 1a regulatory subunit, protein kinase, cAMP-dependent, regulatory subunit type I alpha, protein kinase, cAMP-dependent, regulatory, type I, alpha, tissue-specific extinguisher 1

WB, IHC

Liquid