Proteïne A ELISA KIT

Protein A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Protein A. Samples containing Protein A are first diluted in the Sample Diluent provided with the kits. The Denaturing Buffer is then added and mixed to dissociate the Protein A from the product antibody. The samples are then reacted in microtiter strips coated with a monoclonal anti-Protein A capture antibody. A second anti-Protein A biotinylated monoclonal antibody is simultaneously reacted forming a sandwich complex of solid phase antibody-Protein A: biotin labeled antibody. After a wash step to remove any unbound reactants, Streptavidin labeled HRP is added to each well. After a short incubation period, the strips are then reacted with TMB substrate. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (OD) to the concentration of standards. The Protein A concentration in each sample is interpolated from this standard curve.

Protein A

Colorimetric

Quantitative

Plasma, Serum

0.078-5 ng/mL

80-120%

20 pg/mL

2-8 °C

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.

For research use only.