PTPRR Antilichaam/eiwittyrosinefosfatasereceptor type R

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

Q15256

Human, Mouse, Rat

A synthesized peptide derived from human PTPRR was used as the immunogen for the PTPRR antibody.

29P99

WB, IHC

Affinity-chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This PTPRR antibody is available for research use only.

Store the PTPRR antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Optimal dilution of the PTPRR antibody should be determined by the researcher.

Perinuclear, cell membrane

Western blot analysis of PTPRR using anti-PTPRR antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPRR antibody at 1:500 overnight at 4oC, then washed with TBS-10%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for PTPRR at approximately 70 kDa. The expected band size for PTPRR is at 70 kDa.