PUMA Antilichaam

PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1) . The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2) . PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

27113

Q96PG8

NP_055232

Human, Rat

3043

Unconjugated

Primary Antibodies

Independent Antibody Validation (Figure 2) shows similar PUMA expression profile in both human and mouse cell lines detected by two independent anti-PUMA antibodies that recognize different epitopes, 3041 against C-terminus and 3043 against the N-terminus. PUMA proteins are detected in most of the cell lines with different expression levels by the two independent antibodies. Independent Antibody Validation3041 3043 KO Validation (Figure 7, 8) shows lack of PUMA expression in the thymocytes or neurons of PUMA knockout mice. KO Validation Animal Species Reactivity (Figure 10) shows PUMA expression in the rat retina detected with anti-PUMA antibodies (3043) . Animal Species Reactivity

Apoptosis, Autophagy, Cancer

PUMA Antibody is affinity chromatography purified via peptide column.

Cat. No. 1210 - 293 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Mouse: (78.6%)

PUMA Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

PUMA antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human PUMA has 4 isoforms, including isoform 1 (193aa, 21kD), isoform 2 (131aa, 14kD), isoform 3 (101aa, 10kD) and isofrom 4 (261aa, 27kD) . This antibody detects human isoform 1, and is predicted to detect mouse and rat PUMA (193aa, 21kD for both of them) .

BBC3

Cat. No. 1211 - HepG2 Cell Lysate

Cat. No. 10-001 - Human Breast Tissue Slide

Cat. No. 1204 – K562 Cell Lysate

BCL2 binding component 3

Homo sapiens

Nakano and Vousden. Mol Cell. 2001; 7 (3) 683-94

Han et al. Proc Natl Acad Sci U S A. 2001;98 (20) :11318-23.

18259198

23056511

18604177

22736709

21658600

29686375

27655665

30068931

PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3

ELISA, WB, IHC-P, IF

15193488

Liquid