PXYLP1 Antilichaam / 2-Fosphoxylose fosfatase 1

PXYLP1 dephosphorylates the xylose (xyl) residue in the glycosaminoglycan (GAG) -protein linkage region of proteoglycans, which is required for biosynthetic maturation of the linkage region. Responsible for the 2-O-dephosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature glycosaminoglycan (GAG) chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-dephosphorylation during completion of linkage region formation is a prerequisite for the initiation and efficient elongation of the repeating disaccharide region of GAG chains.

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q8TE99

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids K45-D459) was used as the immunogen for the PXYLP1 antibody.

WB, FACS, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This PXYLP1 antibody is available for research use only.

After reconstitution, the PXYLP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the PXYLP1 antibody should be determined by the researcher.

Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with PXYLP1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= PXYLP1 antibody.