Pyridoxaal kinase Antilichaam/PDXK

Pyridoxal kinase is an enzyme that in humans is encoded by the PDXK gene. The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

O00764

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids H13-E280) was used as the immunogen for the Pyridoxal kinase antibody.

WB, IHC-P, FACS, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Pyridoxal kinase antibody is available for research use only.

After reconstitution, the Pyridoxal kinase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Pyridoxal kinase antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE human colon adenocarcinoma tissue with Pyridoxal kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.