Pyruvate Carboxylase Antilichaam / PC

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

P11498

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids R88-D1165) was used as the immunogen for the Pyruvate Carboxylase antibody.

WB, IHC-P, IF, FACS, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Pyruvate Carboxylase antibody is available for research use only.

After reconstitution, the Pyruvate Carboxylase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Pyruvate Carboxylase antibody should be determined by the researcher.

Cytoplasm

Immunofluorescent staining of FFPE human HeLa cells with Pyruvate Carboxylase antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.