QuicKey Pro Human SEMA4D (Semaforine 4D) ELISA Kit

SEMA4D

Q92854

This kit recognizes SEMA4D in samples.No significant cross-reactivity or interference between SEMA4D and analogues was observed.

CD100; M-sema-G; SEMAJ; coll-4

Sandwich-ELISA

ELISA

Immunology; Neuroscience

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SEMA4D . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SEMA4D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SEMA4D , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 14 nm. The OD value is proportional to the concentration of SEMA4D . You can calculate the concentration of SEMA4D in the samples by comparing the OD of the samples to the standard curve.

1 h 30 min

Serum, plasma and other biological fluids

3.91-250 ng/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

2.34 ng/mL

Ice bag

2-8°C,6 months

https://789.bio/eb/iGi4hTH0

Microplate reader

Human

This ELISA kit applies to the in vitro quantitative determination of Human SEMA4D concentrations in serum, plasma and other biological fluids.