QuicKey Pro Mouse CD48 (Cluster Of Differentiation 48) ELISA Kit

CD48

P18181

This kit recognizes Human RNASE3 in samples. No significant cross-reactivity or interference between Human RNASE3 and analogues was observed.

CD48; BLAST-1; BCM-1; SLAMF2

Sandwich-ELISA

ELISA

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD48. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD48 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD48, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CD48. You can calculate the concentration of Mouse CD48 in the samples by comparing the OD of the samples to the standard curve.

1 h 30 min

Tissue homogenates, cell lysates and other biological fluids

15.63-1000 pg/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

9.38 pg/mL

Ice bag

2-8°C,6 months

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse CD48 concentrations in serum, plasma and other biological fluids.