QuicKey Pro Mouse LAB7-1 (B-Lymfocyten Activation Antigen B7-1) ELISA Kit

LAB7-1

Q00609

This kit recognizes Human CD5L in samples. No significant cross-reactivity or interference between Human CD5L and analogues was observed.

CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1

Sandwich-ELISA

ELISA

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LAB7-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LAB7-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LAB7-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LAB7-1. You can calculate the concentration of Mouse LAB7-1 in the samples by comparing the OD of the samples to the standard curve.

1 h 30 min

Tissue homogenates, cell lysates and other biological fluids

0.39-25 ng/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

0.23 ng/mL

Ice bag

2-8°C,6 months

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse LAB7-1 concentrations in serum, plasma and other biological fluids.