R-Spondin 1 / RSPO1 Proteïne (aa 1-146, zijn label)

RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

CRISTIN3 Protein; FLJ40906 Protein; RSPO Protein; RSPONDIN Protein

NP_001033722.1

Human

Human

His-Tag

See Datasheet send with the product

< 1.0 EU per μg of the protein as determined by the LAL method

> 98 % as determined by SDS-PAGE

1. Measured by its ability to bind recombinant mouse CD36 in a functional ELISA. 2.Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 15-150 ng/mL in the presence of 2 ng/mL recombinant Wnt3a.

137

Lyophilized

PBS, pH 7.4

See Datasheet

23 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells