R-Sponding -1

R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in the adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Recombinant Murine R-Spondin-1 is a 27.1 kDa protein consisting of 245 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions.

Roof plate-specific Spondin-1, RSPO1

192199

Q9Z132

NP_619624.2

NM_138683.2

Mouse

Mouse

SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCQEGLYL HKGRCYPACP EGSTAANSTM ECGSPAQCEM SEWSPWGPCS KKRKLCGFRK GSEERTRRVL HAPGGDHTTC SDTKETRKCT VRRTPCPEGQ KRRKGGQGRR ENANRHPARK NSKEPGSNSR RHKGQQQPQP GTTGPLTSVG PTWAQ

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

< 0.1 ng/µg of protein (< 1EU/µg)

> 95% by SDS-PAGE & HPLC analyses

R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells.

245

Lyophilized

10mM Sodium Phosphate, pH 7.5 and 150mM NaCl

Water

40.0 kDa

CHO cells