RAB27B Antilichaam / Ras-gerelateerd eiwit Rab-27B

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

O00194

Human, Mouse, Rat

A synthetic peptide corresponding to a sequence at the C-terminus of human RAB27B was used as the immunogen for the RAB27B antibody.

WB, IHC, ICC/IF, FACS

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This RAB27B antibody is available for research use only.

After reconstitution, the RAB27B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the RAB27B antibody should be determined by the researcher.

Cytoplasm, Golgi, Plasma membrane

Immunohistochemical staining of RAB27B using anti-RAB27B antibody. RAB27B was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RAB27B antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.