RAC1 Antilichaam

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity) . Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Rac1 has been demonstrated to play a critical role in tumor progression of human colorectal adenocarcinoma cells. In one study, overexpression of Rac1 accelerated the tumorigenic process, whereas Rac1 inhibition completed suppressed tumor formation.

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

P63000

Human

Full length recombinant human protein was used as the immunogen for this RAC1 antibody.

CPTC-RAC1-1

WB, IHC-P

Protein G affinity chromatography

Purified

This RAC1 antibody is available for research use only.

Store the RAC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Optimal dilution of the RAC1 antibody should be determined by the researcher.

Cell membrane, cytoplasm

IHC staining of FFPE human lung with lung antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.