Ramos Lysate

Ramos cells are derived from a Burkitt's lymphoma which does not possess the Epstein Barr Virus (EBV) genome. EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains. Cells are used as model of B lymphocytes and for apoptosis studies.

Human Burkitt's lymphoma cell line

Human

WB

2 mg/mL (determined by BCA assay)

Ramos, ATGL0010-10 µg, ATGL0010-20 µg, ATGL0010-50 µg, ATGL0010-100 µg, ATGL0010-250 µg, ATGL0010-500 µg, ATGL0010-1 mg, ATGL0010-10, ATGL0010-20, ATGL0010-50, ATGL0010-100, ATGL0010-250, ATGL0010-500, ATGL0010-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : Ramos Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody