Ras-GRF1 (fosfo Ser916) Antilichaam

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors) . Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist) -induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

19417

Q13972

NP_002882.3

Mouse, Rat

Ras-GRF1 (phospho Ser916) antibody was raised against a peptide sequence around phosphorylation site of Serine 916 (N-E-G (p) -T P) derived from Human Ras-GRF1.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

145 kDa

IgG

The antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.

Rasgrf1

RAS protein-specific guanine nucleotide-releasing factor 1

Homo sapiens

Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285.

Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189.

Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379.

Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.

CDC25, GNRP, GRF1, RASGRF1

IHC

6755288

Liquid