Raw264,7 Lysaat

Raw264. 7 cells are established from ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV) . This line does not secrete detectable virus particles and is negative in the XC plaque formation assay. Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

Mouse

WB

2 mg/mL (determined by BCA assay)

Raw264.7, ATGL0026-10 µg, ATGL0026-20 µg, ATGL0026-50 µg, ATGL0026-100 µg, ATGL0026-250 µg, ATGL0026-500 µg, ATGL0026-1 mg, ATGL0026-10, ATGL0026-20, ATGL0026-50, ATGL0026-100, ATGL0026-250, ATGL0026-500, ATGL0026-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Mouse

All lanes : Raw264.7 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody