RBM12B Antilichaam/RNA-bindend eiwit 12B

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q8IXT5

Human

E.coli-derived human RBM12B recombinant protein (Position: L186-K997) was used as the immunogen for the RBM12B antibody.

WB, ICC, IF, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This RBM12B antibody is available for research use only.

After reconstitution, the RBM12B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the RBM12B antibody should be determined by the researcher.

Nucleoplasm

Immunofluorescent staining of RBM12B using anti-RBM12B antibody (red) and anti-Beta Tubulin antibody (green) . RBM12B was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-RBM12B antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and FITC Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.