Recombinant aequorea victoria GFP-eiwit

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E. coli and purified by using conventional chromatography.

Green fluorescent protein

E.coli

Aequorea victoria

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

26.8 kDa (238aa) confirmed by MALDI-TOF

GFP, Green fluorescent protein, ATGP0302-10 µg, ATGP0302-20 µg, ATGP0302-50 µg, ATGP0302-100 µg, ATGP0302-250 µg, ATGP0302-500 µg, ATGP0302-1 mg, ATGP0302-10, ATGP0302-20, ATGP0302-50, ATGP0302-100, ATGP0302-250, ATGP0302-500, ATGP0302-1

Millan MI, et al. (2009) . Medicina. 69 (3) :370-4.; ; Marshall J, et al. (1995) . Neuron, 14 (2) :211-5.;

Jo J, et al. Cell-penetrating peptide (CPP) -conjugated proteins is an efficient tool for manipulation of human mesenchymal stromal cells. (Sci Rep. 2014) {https://www.ncbi.nlm.nih.gov/pubmed/24625570}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Fusion Tag & Detection

AAA27721.1

P42212

Aequorea victoria

MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK