Recombinant bacillus Intein-eiwit

Intein-CBD, also known as Intein, is a segment of a protein that is able to excise itself and rejoin the remaining portions with a peptide bond. Most reported inteins also contain an endonuclease domain that plays a role in intein propagation. In fact, many genes have unrelated intein-coding segments inserted at different positions. Since then, inteins have been found in all three domains of life (eukaryotes, bacteria, and archaea) and in viruses. Recombinant Bacillus circulans Intein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Intein-CBD

E.coli

Bacillus

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

59.5 kDa (533aa) confirmed by MALDI-TOF

Intein, Intein-CBD, ATGP1259, ATGP3997-10 µg, ATGP3997-20 µg, ATGP3997-50 µg, ATGP3997-100 µg, ATGP3997-250 µg, ATGP3997-500 µg, ATGP3997-1 mg, ATGP3997-10, ATGP3997-20, ATGP3997-50, ATGP3997-100, ATGP3997-250, ATGP3997-500, ATGP3997-1

Goqarten JP. (2006) BMC Evol Biol. 13:6-94.; ; Anraku Y., et al. (2005) IuBMB Kife. 57:563-74.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Enzymes & Proteases

CAG28939.1

Bacillus