Recombinant BAX Antilichaam

Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Western blot: 2-4 µg/mL

Q07812

Human

A portion of amino acids 1-100 from human BAX protein was used as the immunogen for the recombinant BAX antibody.

RBAX/962

WB

Protein G affinity

Purified

This recombinant BAX antibody is available for research use only.

Aliquot the recombinant BAX antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant BAX antibody should be determined by the researcher.

Cytoplasm

Western blot testing of human A549 and MCF7 cell lysate with recombinant Bax antibody (clone rBAX/962) . Predicted molecular weight ~21 kDa.