Recombinant BCL2L4 Antilichaam/BAX

Recognizes a protein of 21 kDa, identified as the Bax protein (also called Bcl-2-like protein 4 or BCL2L4) . This MAb is highly specific to BCL2L4 and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. BCL2L4 has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of BCL2L4 accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and BCL2L4 also counters the death repressor activity of Bcl-2.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

Q07812

Human

A portion of amino acids 1-100 from human BAX protein was used as the immunogen for the recombinant BCL2L4 antibody.

BAX/9925R

IHC-P, WB

Purified

This recombinant BCL2L4 antibody is available for research use only.

Aliquot the recombinant BCL2L4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant BCL2L4 antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE human liver tissue with recombinant BCL2L4 antibody (clone BAX/9925R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.