Recombinant BRCA1 Antilichaam / Borstkanker type 1 gevoeligheidseiwit

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC) . This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P38398

Human

A portion of the N-terminal 304 amino acids of human BRCA1 protein was used as the immunogen for the recombinant BRCA1 antibody.

BRCA1/9797R

IHC-P

Purified

This recombinant BRCA1 antibody is available for research use only.

Aliquot the recombinant BRCA1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant BRCA1 antibody should be determined by the researcher.

Chromosome, Cytoplasm, Nucleus

IHC staining of FFPE human breast carcinoma tissue with BRCA1 antibody (clone BRCA1/9797R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.