Recombinant canine IL-1 alfa/IL1A eiwit

IL-1 alpha /IL-1F1, also known as hematopoietin 1 is a cytokine of the interleukin 1 family. IL-1α is a unique member in the cytokine family in the sense that the structure of its initially synthesized precursor does not contain a signal peptide fragment. In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1 alpha inhibitors are being developed to interrupt those processes and treat diseases. IL-1 alpha is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. Recombinant canine IL-1 alpha /IL-1F1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Canine IL-1 alpha/IL-1F1 in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 2 ng/mL.

IL-1 alpha, Il-1a, Interleukin 1 alpha, Interleukin-1 alpha precursor

Baculovirus

Canine

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS - PAGE

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 2 ng/mL.

19.3kDa (166aa)

IL 1 alpha, IL1 alpha, IL-1 alpha, Il-1a, Il1a, Il 1a, Interleukin 1 alpha, Interleukin-1 alpha precursor, ATGP3976-10 µg, ATGP3976-20 µg, ATGP3976-50 µg, ATGP3976-100 µg, ATGP3976-250 µg, ATGP3976-500 µg, ATGP3976-1 mg, ATGP3976-010, ATGP3976-020, ATGP3976-050, ATGP3976-100, ATGP3976-250, ATGP3976-500, ATGP3976-01M

Watanabe N., et al, (1994) Cytokine. 6:597–601.; ; Nicklin MJ., et al, (1994) Genomics. 19:382–384.; ; March CJ, Mosley B., et al, (1985) . Nature. 315 : 641–647.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_001003157

O46612

Canine

Ala

SVAYNFH NNEKYNYIRI IKSQFILNDN LNQSIVRQTG GNYLMTAALQ NLDDAVKFDM GAYTSEDSKL PVTLRISKTR LFVSAQNEDE PVLLKEMPET PKTIRDETNL LFFWERHGSK HYFKSVAQPK LFIATQERKL VHMARGQPSI TDFRLLETQP