Recombinant canine IL-1 bèta/IL1B eiwit

Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. Recombinant canine IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques.

Canine IL-1B stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range range ≤ 5pg/ml.

Interleukin-1 beta, IL-1 beta

E.coli

Canine

Non-Tagged

SDS-PAGE, Bioactivity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 5pg/ml.

17.5 kDa (153aa) confirmed by MALDI-TOF

Beta proprotein, Catabolin, IL 1, IL 1 b, IL 1b, IL 1beta, IL 1 beta, IL1, IL-1, IL1 b, IL1 beta, IL1b, IL-1b, IL1-beta, IL-1beta, IL-1-beta, IL1F2, Interleukin 1, Interleukin 1 b, Interleukin 1 beta, Interleukin 1b, Interleukin 1beta, ATGP3956-10 µg, ATGP3956-20 µg, ATGP3956-50 µg, ATGP3956-100 µg, ATGP3956-250 µg, ATGP3956-500 µg, ATGP3956-1 mg, ATGP3956-010, ATGP3956-020, ATGP3956-050, ATGP3956-100, ATGP3956-250, ATGP3956-500, ATGP3956-01M

Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629; ; Curr Pharm Des. (2017), 23 (32) :4893-4905.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Hormones & Cytokines

NP_001033060.1

Q2VCE2

Canine

MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS