Recombinant canine IL-2 (C147S) eiwit

IL2, also known as Interleukin2, is an immunoregulatory lymphokine produced by T-cell in response to antigenic or mitogenic stimulation IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated kill cells, monocytres, macrophages and oligodendrocytes. Recombinant canine IL2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Canine IL-2 stimulates cell cytotoxicity in the CTLL-2 mouse cytotoxic T cell. The ED50 range ≤ 0.3 ng/mL.

Interleukin-2, IL-2, T-cell growth factor, TCGF

E.coli

Canine

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 range ≤ 0.3 ng/mL.

18.1 kDa (160aa) confirmed by MALDI-TOF

IL2 (C147S), Interleukin-2, Interleukin2, Interleukin 2, IL-2, IL 2, ATGP3544-10 µg, ATGP3544-20 µg, ATGP3544-50 µg, ATGP3544-100 µg, ATGP3544-250 µg, ATGP3544-500 µg, ATGP3544-1 mg, ATGP3544-010, ATGP3544-020, ATGP3544-050, ATGP3544-100, ATGP3544-250, ATGP3544-500, ATGP3544-01M

Nedoszytko B., et al. (2016) Postepy Dermatol Alerqol. 33 (6) : 429-434.; ; Michael HT., et al. (2013) Vet Immunol Immunopathol. 155 (3) : 211-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Hormones & Cytokines

NP_001003305

Q29416

Canine

APITS SSTKETEQQM EQLLLDLQLL LNGVNNYENP QLSRMLTFKF YTPKKATEFT HLQCLAEELK NLEEVLGLPQ SKNVHLTDTK ELISNMNVTL LKLKGSETSY NCEYDDETAT ITEFLNKWIT FSQSIFSTLT