Recombinant canine IL-21 eiwit

IL-21, also known as Interleukin-21, is an important modulator of natural killer (NK) cell function. However, little is known about IL-21 function in canine NK cells because the phenotype of these cells remains undefined. NK cells proliferated rapidly in response to activation by IL-21 for 3 weeks, and IL-21 was able to induce changes in the mRNA expression of NK cell-related receptors and enhance the effector function of NK cells in perforin- and granzyme-B-dependent manners. The duration, frequency and timing of IL-21 stimulation during culture affected the rate of proliferation, patterns of receptor expression, cytokine production, and anti-tumor activity. It has a potential predicating that synergistic interactions of IL-21 with IL-2 and IL-15 play an important role in the proliferation, receptor expression, and effector function of canine NK cells. Recombinant canine IL-21, was expressed in E. coli and purified by using conventional chromatography techniques.

Canine IL-21 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤ 2 ng/mL.

Interleukin-21

E.coli

Canine

Non-Tagged

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤ 2 ng/mL.

15 kDa (130aa), confirmed by MALDI-TOF

, ATGP3974-10 µg, ATGP3974-20 µg, ATGP3974-50 µg, ATGP3974-100 µg, ATGP3974-250 µg, ATGP3974-500 µg, ATGP3974-1 mg, ATGP3974-10, ATGP3974-20, ATGP3974-50, ATGP3974-100, ATGP3974-250, ATGP3974-500, ATGP3974-1

DJ Shin., et al. (2015) Vet Immunol Immunopathol. 15;165 (1-2) :22-33.; ; Gui G., et al. (2017) Clin Immunol. 183:266-272.; ; Parrish-Novak J., et al. (2000) Nature. 408 (6808) :57-63.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Immunology

NP_001003347.1

Q6L7I9

Canine

MHKSSFQEQD LLLIRMRQLI DIVDQLKNYV NDLDPESLPA PEDVKRHCER SAFSCFQKVQ LKAANTGGNE QIINVLTKQL KRKLPPTNAG RRQKHRPACP SCDSYEKAPP KEFLERLKSL IQKMIHQHLS