Recombinant canine IL-5 eiwit

IL-5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunoglobulin secretion. It related hematopoietic cytokines that are important for allergic inflammation. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. Recombinant canine IL-5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques

Canine IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 15 ng/mL.

Interleukin-5, Eosinophil differentiation factor, T-cell replacing factor, TRF, EDF

Baculovirus

Canine

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS - PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 15 ng/mL.

13.9kDa (119aa)

B cell growth factor II, B-cell growth factor II, BCGF I, BCGF II, BCGFI, BCGF-I, BCGFII, BCGF-II, EDF, Eosinophil differentiation factor, IL 5, IL5, IL-5, Interleukin 5, Interleukin5, Interleukin-5, T cell replacing factor, T-cell replacing factor, TRF, ATGP3985-10 µg, ATGP3985-20 µg, ATGP3985-50 µg, ATGP3985-100 µg, ATGP3985-250 µg, ATGP3985-500 µg, ATGP3985-1 mg, ATGP3985-010, ATGP3985-020, ATGP3985-050, ATGP3985-100, ATGP3985-250, ATGP3985-500, ATGP3985-01M

Martinez-Moczygemba M., et al. (2003) J Allergy Clin Immunol. 112:653-665.; ; Milburn MV., et al. (1993) Nature. 363:172-176.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology, Cancer

NP_001006951

Q95J76

Canine

Val 22