Recombinant CD34 Antilichaam

This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P28906

Human

Blast cells from a chronic myeloid leukemia patient were used as the immunogen for this recombinant CD34 antibody.

RICO-115

IHC-P

Protein G affinity

Purified

This recombinant CD34 antibody is available for research use only.

Aliquot the recombinant CD34 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant CD34 antibody should be determined by the researcher.

Cell surface

IHC staining of FFPE human uterus tissue with recombinant CD34 antibody (clone rICO-115) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.