Recombinant CDKN1B Antilichaam / p27Kip1

This MAb recognizes a 27kDa protein called p27Kip1/CDKN1B/Cyclin-dependent kinase inhibitor 1B, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

P46527

Human

A recombinant human CDKN1B protein was used as the immunogen for the recombinant CDKN1B antibody.

RCDKN1B/13245

IHC-P, WB

Protein G affinity

Purified

This recombinant CDKN1B antibody is available for research use only.

Aliquot the recombinant CDKN1B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant CDKN1B antibody should be determined by the researcher.

Cytoplasm, Endosome, Nucleus

Western blot testing of human SK-BR-3 cell lysate with recombinant CDKN1B antibody (clone rCDKN1B/13245) . Commonly observed at ~27 kDa.