Recombinant cinate IL-2 (C146S) eiwit

IL2, also known as interleukin2, is an immunoregulatory lymphokine produced by T-cell in response to antigenic or mitogenic stimulation IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated kill cells, monocytes, macrophages and oligodendrocytes. Recombinant feline IL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Feline IL-2 (C146S) stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.3 ng/mL.

Interleukin-2, IL-2, T-cell growth factor, TCGF

E.coli

Feline

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by BCA assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.3 ng/mL.

17.8 kDa (157aa) Confirmed by MALDI-TOF

IL 2, IL2, IL-2, Interleukin 2, Interleukin2, Interleukin-2, T cell growth factor, T-cell growth factor, TCGF, ATGP3611-10 µg, ATGP3611-20 µg, ATGP3611-50 µg, ATGP3611-100 µg, ATGP3611-250 µg, ATGP3611-500 µg, ATGP3611-1 mg, ATGP3611-010, ATGP3611-020, ATGP3611-050, ATGP3611-100, ATGP3611-250, ATGP3611-500, ATGP3611-01M

Taniguchi T., et al. (1983) . Nature. 302 (5906) : 305-10; ; Nedoszytko B., et al. (2016) Postepy Dermatol Alerqol. 33 (6) : 429-434

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol

Hormones & Cytokines

NP_001036802

Q07885

Feline

APASSST KETQQQLEQL LLDLRLLLNG VNNPENPKLS RMLTFKFYVP KKATELTHLQ CLVEELKPLE EVLYLAQSKN FHLNHIKELM SNINVTVLKL KGSETRFTCN YDDETATIVE FLNKWITFCQ SIFSTLT