Recombinant CNTF-eiwit van ratten

CNTF also known as ciliary neurotrphic factor is a survival factor for various neuronal cell types. CNTF is highly conserved across species and exhibits cross-species bioactivity. CNTF is a 25 kDa polypeptide that was originally isolated as a target-derived survival factor of parasympathetic ciliary ganglion neurons. Binding of CNTF to CNTF receptor triggers heterodimerization of gp130 and LIFR, forming an active trimeric receptor complex and activates the downstream signaling pathway. Recombinant rat CNTF, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Rat CNTF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤40 ng/mL.

E.coli

Rat

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤40 ng/mL.

25.2 kDa (223aa) confirmed by MALDI-TOF

CNTF, ATGP3219-10 µg, ATGP3219-20 µg, ATGP3219-50 µg, ATGP3219-100 µg, ATGP3219-250 µg, ATGP3219-500 µg, ATGP3219-1 mg, ATGP3219-10, ATGP3219-20, ATGP3219-50, ATGP3219-100, ATGP3219-250, ATGP3219-500, ATGP3219-1

Linker R., et al. (2009) Crit Rev Immunol. 29 (1) :43-68.; ; Stahl N., et al. (1994) J Neurobiol. 25 (11) :1454-66.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_037298

P20294

Rat

MAFAEQT PLTLHRRDLC SRSIWLARKI RSDLTALMES YVKHQGLNKN INLDSVDGVP VASTDRWSEM TEAERLQENL QAYRTFQGML TKLLEDQRVH FTPTEGDFHQ AIHTLMLQVS AFAYQLEELM VLLEQKIPEN EADGMPATVG DGGLFEKKLW GLKVLQELSQ WTVRSIHDLR VISSHQMGIS ALESHYGAKD KQM