Recombinant Cycline B1 Monoklonale Antilichaam

Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF) . MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis.

Cyclin B1

P14635

Human

Recombinant human Cyclin B1 fragment

G2 mitotic specific cyclin B; G2/mitotic-specific cyclin-B; Cyclin B; CCNB; CCNB1; Cyclin B1; CCNB 1; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1

A546

Unconjugated

WB; IF; IP

Cell Biology; Epigenetics and Nuclear Signaling; Cancer

Protein A purified

1 mg/mL

WB 1:500-1:1000; IF 1:50; IP 1:50-1:100

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Ice bag

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

https://789.bio/eb/fkUi12I7

55 kDa

55 kDa

IgG, κ