Recombinant dengue virus 2 Envelop eiwit

Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. This protein is synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes. Recombinant Dengue virus DENV protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dengue virus 2 envelope protein

E.coli

Dengue virus 2

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

13.8 kDa (126aa)

Envelope, ATGP2755-10 µg, ATGP2755-20 µg, ATGP2755-50 µg, ATGP2755-100 µg, ATGP2755-250 µg, ATGP2755-500 µg, ATGP2755-1 mg, ATGP2755-10, ATGP2755-20, ATGP2755-50, ATGP2755-100, ATGP2755-250, ATGP2755-500, ATGP2755-1

Blok J., et al. (1989) Arch. Virol. 105:39-53

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Virus

AAA42954

Q88631

Dengue virus 2

SYSMCTG KFKVVKEIAE TQHGTIVIRV QYEGDGSPCK IPFEIMDLEK RHVLGRLITV NPIVTEKDSP VNIEAEPPFG DSYIIIGVEP GQLKLNWFKK GSSIGQ