Recombinant E.coli Tryptofaan Synthase β Chain/Trp B Proteïne (His Tag)

Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C- (indol-3-yl) glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.

Tryptophan Synthase β Chain; Trp B

Tryptophan synthase beta chain; trpB

P0A879

P0A879

E.coli

N-His

Thr2-Ile397

< 1.0 EU per μg of the protein as determined by the LAL method.

> 95 % as determined by reducing SDS-PAGE.

Not validated for activity

Not Applicable

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

https://789.bio/eb/nDabz1

43.8 kDa

38-48 kDa

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 0.05% Tween 80, pH 8.5.

E.coli