Recombinant e.coli ackA-eiwit

AckA catalyzes the formation of acetyl phosphate from acetate and ATP. This protein can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. Recombinant E. coli ackA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Acetate kinase A and propionate kinase 2, ECK2290, JW2293

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

45.7 kDa (423aa) confirmed by MALDI-TOF

AckA, Acetate kinase A and propionate kinase 2, ECK2290, JW2293, ATGP2844-10 µg, ATGP2844-20 µg, ATGP2844-50 µg, ATGP2844-100 µg, ATGP2844-250 µg, ATGP2844-500 µg, ATGP2844-1 mg, ATGP2844-10, ATGP2844-20, ATGP2844-50, ATGP2844-100, ATGP2844-250, ATGP2844-500, ATGP2844-1

Blattner, F.R., et al. (1997) Science 277 (5331), 1453-1462.; ; Riley, M., et al. (2006) Nucleic Acids Res. 34 (1), 1-9

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Enzymes & Proteases

NP_416799

P0A6A3

E.coli

MGSSHHHHHH SSGLVPRGSH MGSMSSKLVL VLNCGSSSLK FAIIDAVNGE EYLSGLAECF HLPEARIKWK MDGNKQEAAL GAGAAHSEAL NFIVNTILAQ KPELSAQLTA IGHRIVHGGE KYTSSVVIDE SVIQGIKDAA SFAPLHNPAH LIGIEEALKS FPQLKDKNVA VFDTAFHQTM PEESYLYALP YNLYKEHGIR RYGAHGTSHF YVTQEAAKML NKPVEELNII TCHLGNGGSV SAIRNGKCVD TSMGLTPLEG LVMGTRSGDI DPAIIFHLHD TLGMSVDAIN KLLTKESGLL GLTEVTSDCR YVEDNYATKE DAKRAMDVYC HRLAKYIGAY TALMDGRLDA VVFTGGIGEN AAMVRELSLG KLGVLGFEVD HERNLAARFG KSGFINKEGT RPAVVIPTNE ELVIAQDASR LTA