Recombinant e.coli ampC/Beta-lactamase-eiwit

AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E. coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

AmpC, ampA, Cephalosporinase, D-alanine carboxypeptidase

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

41.8 kDa (379aa) confirmed by MALDI-TOF

Ampb-lactamase, ampC, ampA, Cephalosporinase, D-alanine carboxypeptidase, ATGP0942-10 µg, ATGP0942-20 µg, ATGP0942-50 µg, ATGP0942-100 µg, ATGP0942-250 µg, ATGP0942-500 µg, ATGP0942-1 mg, ATGP0942-10, ATGP0942-20, ATGP0942-50, ATGP0942-100, ATGP0942-250, ATGP0942-500, ATGP0942-1

Jaurin B., et al. (1981) Proc. Natl. Acad. Sci. u.S.A. 78:4897-4901; ; Morandi F, et al. (2003) J AM Chem Soc. 125 (3) :685-95

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_418574

P00811

E.coli

MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ